STUDY OF BACTERIAL RESISTANCE TO ORGANOPHOSPHOROUS PESTICIDES IN IRAN
Abstract
The broadness application of organophosphorus compounds has abounded the number of its
polluted areas. Bioremediation has widely focused on insitu bacterial degradation of
organophosphorus residues in the world. Therefore, in this research six numbers of samples
from two different sources, soil and water randomly were isolated using different
organophosphorus pesticides containing mineral solution without supplementation. More than
100 isolated strains were selected according to their simultaneous optimal growth on mineral
medium with organophosphorus and Mac Conkey,s agar. More than 50 percent of them were
lost above resistance. The resistant strains were identified by two methods, the biochemical
convention and API 20E procedure with positive agreement. The identified strains belonged to
Pseudomonas and Flavobacterium species. The maximum tolerant concentrations of different
organophosphorus pesticides by these resistant strains were 2.5, 4 and 8 g/L of guthion, methyl parathion and Dimethoate, respectively. The resistance to these pesticides due to organ phosphorous degrading plasmids had the ability to express hydrolytic enzymes. Resistant bacteria lost these plasmids by acridin orange and could translocate to sensitive strains. Thus, certain environmental bacteria could be used as protection tools against antinerve agents.
polluted areas. Bioremediation has widely focused on insitu bacterial degradation of
organophosphorus residues in the world. Therefore, in this research six numbers of samples
from two different sources, soil and water randomly were isolated using different
organophosphorus pesticides containing mineral solution without supplementation. More than
100 isolated strains were selected according to their simultaneous optimal growth on mineral
medium with organophosphorus and Mac Conkey,s agar. More than 50 percent of them were
lost above resistance. The resistant strains were identified by two methods, the biochemical
convention and API 20E procedure with positive agreement. The identified strains belonged to
Pseudomonas and Flavobacterium species. The maximum tolerant concentrations of different
organophosphorus pesticides by these resistant strains were 2.5, 4 and 8 g/L of guthion, methyl parathion and Dimethoate, respectively. The resistance to these pesticides due to organ phosphorous degrading plasmids had the ability to express hydrolytic enzymes. Resistant bacteria lost these plasmids by acridin orange and could translocate to sensitive strains. Thus, certain environmental bacteria could be used as protection tools against antinerve agents.
Keywords
Bacterial resistance, organophosphorous pestisides, antinerve agents,
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