COMBINED EFFECT OF WATER CONTAMINATION WITH COBALT AND NICKEL ON METABOLISM OF ALBINO (WISTAR) RATS

Abstract

The contamination of water by metal compounds is a worldwide environmental problem. Concentration of metals is widely related to biochemical values, which are used in diseases diagnosis due to environmental toxicity. The sub-chronic combined effects of nickel and cobalt on body weight gain and biochemical parameters were determined and compared with those of Ni (2+) or Co (2+) alone in 6 weeks male albino (Wistar) rats. Animals were given drinking solutions of NiSO4 6H2O [Ni (II) cation, LD50/2] or CoSO4 6H2O [Co (II) cation, LD50/2]. For the combined treatment (Ni + Co), the rats received both Ni (II) cation (LD 50/2) and CO (II) cation (LD50/2). Nickel and cobalt treatment decreased body weight gain. The nickel sulphate increased also the glucose level. The two heavy elements produced hepatic and renal damage, characterized by increased activity of alanine and aspartate transaminases (GPT, GOT) and alkaline phosphatase. However lactate dehydrogenase activity (LDH) was decreased. In addition, serum urea, serum creatinine, serum total protein and serum bilurebin concentrations were significantly elevated. In general the combined effect of Ni-Co was slightly less toxic than nickel or cobalt alone, suggesting antagonism between these toxicants.